A Clinostat — Can You Confuse a Plant About Which Way Is Down?
Put a plant on its side and something rather remarkable happens.
It does not simply continue growing sideways.
Within hours, the shoot begins to curve upwards while the root turns in almost exactly the opposite direction.
The plant has no eyes.
It has no ears.
It has no brain.
And yet somehow it appears to know which way is up.
That raises a wonderful biological question:
How does a plant know which way gravity is acting?
One of the classic ways of investigating this is with a wonderfully simple piece of scientific apparatus called a clinostat.
A clinostat slowly rotates a plant so that the direction of gravity is continually changing relative to the plant's tissues.
Gravity has not disappeared.
Instead, from the plant's point of view, there is no longer one consistent direction that remains "down".
Can we confuse the plant's normal gravitational response?
That makes the clinostat a fascinating experiment for anyone interested in plant biology, hormones, tropisms or even how scientists investigate plants in space.
Plants Are Constantly Sensing Their Environment
Plants may look passive, but biologically they are anything but.
They constantly respond to their surroundings.
Among the most familiar responses are:
phototropism — growth in response to light;
gravitropism — growth in response to gravity;
hydrotropism — growth in response to water;
thigmotropism — growth in response to touch.
Gravitropism is particularly interesting because gravity is always present.
A seed germinating underground cannot necessarily use light to decide which direction its new root should grow.
Yet its first root generally grows downwards while its shoot grows upwards.
That is enormously important.
Roots growing downwards are more likely to enter the soil where they can obtain water and mineral ions.
Shoots growing upwards are more likely eventually to reach the light required for photosynthesis.
Plants therefore show two different gravitational responses.
Roots generally have positive gravitropism because they grow towards the direction of gravity.
Shoots generally have negative gravitropism because they grow away from it.
But how can we demonstrate this?
Experiment One: Put the Seedlings on Their Side
The first experiment does not need a clinostat at all.
Grow several seedlings vertically until their young roots and shoots are clearly visible.
Suitable plants might include:
broad beans;
peas;
cress;
radish;
wheat;
oats;
mung beans.
Seeds can be germinated between moist paper, in transparent bags, on agar or in suitable growing medium.
Once the roots and shoots have developed, turn the seedlings through approximately 90 degrees so that they are growing horizontally.
Then watch.
A useful experiment might photograph the seedlings every hour or every few hours.
Over time the shoot should begin curving upwards.
The root should begin curving downwards.
The interesting part is that the plant has not been physically bent by gravity like a piece of soft wire.
Instead, different parts of the growing region have grown at different rates.
The curvature is being produced biologically.
Now Introduce the Clinostat
A clinostat changes the experiment.
Instead of leaving the seedling in one position, attach it to a slowly rotating platform.
The rotation needs to be slow and steady.
The aim is not to spin the plant rapidly.
A simple educational clinostat might rotate at only a few revolutions per minute.
As the plant rotates, gravity is always pulling vertically downwards relative to the room.
But relative to the plant, the apparent direction of gravity continuously changes.
At one moment one side of the plant faces downwards.
Half a rotation later, the opposite side faces downwards.
Over time the plant receives no persistent gravitational direction from one side.
This provides a fascinating comparison.
A Simple Experimental Design
You could prepare three groups of similar seedlings.
Group A — Normal vertical seedlings
Leave these growing normally.
They provide a reference showing ordinary root and shoot development.
Group B — Horizontal stationary seedlings
Place these on their sides and leave them stationary.
These should demonstrate the normal gravitropic response.
The shoots should curve upwards.
The roots should curve downwards.
Group C — Horizontal seedlings on the clinostat
Place comparable seedlings horizontally on the rotating clinostat.
Now photograph and measure their growth.
The question becomes:
Will they curve in the same way as the stationary seedlings?
Ideally the clinostat seedlings should show much less consistent curvature because the gravitational stimulus is continually being reoriented.
That difference is the heart of the experiment.
What Should We Measure?
Simply looking at the seedlings is interesting.
Measuring them turns the demonstration into an investigation.
Photograph each seedling from the same position at regular intervals.
You could record:
shoot length;
root length;
angle of shoot growth;
angle of root growth;
time before curvature becomes visible;
amount of curvature after 12, 24, 48 or 72 hours.
A printed grid placed behind the seedlings can make measurements easier.
Even better, take photographs with the camera fixed in the same position.
Students could then use image-analysis software to estimate the angle through which the root or shoot has curved.
For example, the original direction of growth could be defined as 0 degrees.
If the shoot eventually bends upwards through approximately 70 degrees, that can be compared quantitatively with a clinostat-grown shoot that perhaps changes direction only slightly.
Suddenly a plant on a rotating disc has become a proper experimental investigation.
Keep the Variables Under Control
Clinostat experiments also provide an excellent lesson in experimental design.
If we are investigating gravity, we do not want another directional stimulus dominating the experiment.
Light is the obvious problem.
Shoots also respond strongly to directional light.
If your seedlings are illuminated strongly from one side, you may think you are observing gravitropism when you are actually observing phototropism.
Ideally the illumination should therefore be:
diffuse;
symmetrical;
from directly above where appropriate;
or excluded during the relevant part of the experiment.
Temperature should also be similar between the rotating and stationary seedlings.
The seedlings should ideally be:
the same species;
approximately the same age;
at similar stages of germination;
supplied with similar amounts of water;
exposed to similar temperatures.
The only major difference should be the rotation.
This is precisely the sort of thinking that turns an interesting demonstration into good science.
But How Does the Plant Detect Gravity?
This is where the experiment becomes even more interesting.
Inside certain specialised plant cells are structures containing dense starch-filled organelles called amyloplasts.
When they are involved in gravity sensing, these structures are often described as statoliths.
Because they are relatively dense, they tend to settle towards the lower part of the cell under gravity.
Imagine a snow globe.
Turn the globe sideways and the particles eventually settle towards the new bottom.
Something conceptually similar occurs inside gravity-sensing cells in plants.
The position of these sedimenting statoliths provides information about the direction of gravity.
Specialised gravity-sensing cells are known as statocytes.
In roots, particularly important statocytes occur in the root cap.
In shoots, gravity sensing involves specialised tissues including cells associated with the endodermis.
The movement of the statoliths appears to initiate signalling processes that eventually affect growth.
Detecting Gravity Is Only the Beginning
Knowing which direction gravity acts is not enough.
The plant must somehow turn that information into directional growth.
This brings us to the plant hormone auxin.
When a plant organ is placed horizontally, gravity sensing contributes to an unequal distribution of auxin between the upper and lower sides.
The effects differ between roots and shoots.
In shoots, increased auxin on the lower side generally promotes greater cell elongation.
The lower side therefore grows faster than the upper side.
The shoot curves upwards.
In roots, higher auxin concentrations on the lower side inhibit elongation more strongly.
The upper side therefore elongates faster.
The root curves downwards.
That difference is worth emphasising.
Students sometimes learn the oversimplified rule:
"Auxin makes plants grow."
The real biology is more interesting.
The effect of auxin depends upon:
its concentration;
the plant tissue;
developmental conditions;
interactions with other signalling systems.
The same redistribution of a hormone can therefore contribute to opposite-looking responses in roots and shoots.
What Is the Clinostat Actually Doing?
There is an important scientific caution here.
A clinostat does not switch gravity off.
Gravity is still acting on the plant.
The Earth has not stopped pulling on it.
Instead, the rotation continually changes the direction from which the plant experiences the gravitational stimulus.
If the rotation is appropriate, there is no persistent gravitational direction relative to the plant.
Scientists sometimes describe this as gravity-vector averaging.
That distinction matters because clinostats are sometimes loosely described as producing "zero gravity".
They do not.
Real microgravity requires very different conditions, such as those experienced aboard an orbiting spacecraft.
Clinostats can nevertheless be extremely useful for investigating how organisms respond when they are denied a stable gravitational direction.
More sophisticated research may use devices such as random positioning machines or specialised centrifuge systems.
But the basic scientific idea can be explored with a remarkably simple rotating platform.
Could You Build Your Own Clinostat?
Yes.
A basic educational clinostat does not have to be an expensive scientific instrument.
The essential requirement is a slowly rotating mounting system.
Possible approaches include:
a geared low-speed electric motor;
a small turntable mechanism;
a modified rotating display stand;
a motor controlled using an Arduino or Raspberry Pi;
a 3D-printed support attached to a suitable low-speed motor.
The seedling container needs to be held securely so that it rotates with the axis of the clinostat.
It is important that the seedling does not repeatedly fall or move around inside the container.
The rotation should also be reasonably smooth.
Very rapid rotation creates an additional problem: centrifugal effects.
The objective is therefore not:
Spin the plant as fast as possible.
It is:
Change its orientation slowly enough that gravity does not remain acting in one consistent direction relative to the plant.
That is a much more subtle experiment.
An Excellent Use for Time-Lapse Photography
This experiment is almost perfect for time-lapse photography.
Plants move too slowly for us to appreciate their behaviour easily in real time.
Take one photograph every few minutes and combine the images into a video.
A process taking two days can then be compressed into perhaps 20 or 30 seconds.
The stationary horizontal seedling may appear dramatically to sweep its shoot upwards.
The root moves in the opposite direction.
The clinostat-grown plant may behave very differently.
Time-lapse transforms something that appears static into something almost animal-like.
It is one of the best ways of reminding students that plants are actively responding organisms.
Take the Experiment Further
Once the basic experiment works, there are plenty of possible extensions.
Does the speed of clinostat rotation matter?
Try several rotation speeds.
At what point is the gravitational response most effectively disrupted?
Be careful: very high speeds may introduce centrifugal effects and other mechanical stresses.
Do roots and shoots respond equally quickly?
Measure how long it takes before curvature becomes visible.
Does the root begin responding before the shoot?
Do different plants respond differently?
Compare:
peas;
beans;
cereals;
cress;
radish.
Are the rates of gravitropic response similar?
Does seedling age matter?
Compare very young seedlings with slightly older plants.
What happens after removing the plant from the clinostat?
Allow a plant to rotate for perhaps 24 or 48 hours.
Then stop the clinostat and leave the plant horizontally.
How quickly does gravitropic curvature return?
This gives a wonderfully clear demonstration that the plant's gravity-sensing mechanism is still functioning.
Gravity and Plants in Space
The clinostat experiment naturally leads to a much bigger question.
What happens to plants in space?
If humans are ever to live for long periods:
aboard space stations;
on the Moon;
on Mars;
or during long journeys through the Solar System,
growing plants may become extremely important.
Plants could provide:
food;
oxygen;
carbon dioxide removal;
water recycling;
psychological benefits for crews.
But plants evolved under Earth's gravity.
Remove or greatly reduce that familiar gravitational cue and their normal growth patterns may change.
Space biology therefore asks questions remarkably similar to those we are investigating with our small rotating seedlings:
How important is gravity to plant development?
Can other environmental signals take over?
How do roots decide where to grow when "down" is no longer obvious?
A small clinostat on a classroom or laboratory bench therefore connects remarkably well with experiments conducted in space.
The Bigger Lesson: Plants Are Not Passive
One reason I like experiments such as this is that they change the way we look at plants.
A seedling sitting in a pot can appear to be doing almost nothing.
In reality it is continually:
detecting light;
detecting gravity;
responding to water;
regulating hormones;
changing patterns of cell growth;
directing roots and shoots towards favourable environments.
The plant has no nervous system telling it what to do.
Instead, environmental information is translated into chemical and cellular responses.
That makes a simple question such as:
"Which way is down?"
far more biologically interesting than it first appears.
A Three-to-Seven-Day Experiment That Opens Up a Huge Area of Biology
The clinostat experiment does not need spectacular chemicals, expensive sensors or complicated preparation.
You need seedlings, some careful controls and a slowly rotating platform.
Yet from that simple equipment you can explore:
gravitropism;
positive and negative tropisms;
plant hormones;
auxin redistribution;
differential cell elongation;
root and shoot physiology;
statocytes;
statoliths;
amyloplasts;
experimental controls;
time-lapse photography;
and even plant biology in space.
Most importantly, it encourages exactly the sort of question that good science should encourage.
Put a seedling on its side and it turns.
Rotate it continuously and its behaviour changes.
So the question is no longer simply:
"Do plants respond to gravity?"
We know that they do.
The more interesting question is:
How can an organism with no brain, no eyes and no sense of balance work out which way is down?
Sometimes an experiment does not need to produce an unexpected result to be fascinating.
Sometimes the fascinating part is discovering just how much biology is hidden inside something we normally take completely for granted.


