Making Glue from Milk — The Chemistry of Casein, Coagulation and Precipitation
Milk does not immediately suggest itself as a building material.
We pour it over cereal, add it to tea and coffee, turn it into yoghurt and cheese, and perhaps occasionally forget about it at the back of the fridge.
But hidden inside milk is a substance that can be separated, treated and turned into something quite unexpected:
glue.
The key ingredient is casein, the main family of proteins found in cow's milk.
By adding acid to milk, we can make those proteins come out of suspension as solid curds. Separate those curds, remove as much liquid as possible, and then treat the casein appropriately, and we can produce a surprisingly effective adhesive.
It is an excellent experiment because what initially looks like a simple kitchen activity opens the door to some serious chemistry.
We encounter:
proteins;
acids;
pH;
electrical charge on molecules;
colloids;
precipitation;
coagulation;
filtration;
neutralisation;
polymers;
intermolecular forces;
and the science of adhesives.
It also provides a wonderful reminder that chemistry is not simply about producing coloured solutions in test tubes.
Sometimes chemistry produces useful materials.
Milk Is Much More Complicated Than It Looks
At first glance, milk appears to be a simple white liquid.
Chemically, however, it is a remarkably complicated mixture containing:
water;
proteins;
fats;
lactose;
calcium compounds;
vitamins;
minerals;
and many other dissolved or suspended substances.
Approximately 80% of the protein in cow's milk is casein.
But casein is not simply floating around as individual protein molecules.
Much of it is organised into tiny structures called casein micelles.
These microscopic particles remain dispersed through the water in milk, contributing to its familiar white appearance.
Under normal conditions, the micelles repel one another sufficiently to remain dispersed.
Change the chemistry of their surroundings, however, and that stability can disappear.
That is exactly what we are going to do.
The Key Idea — Make the Casein Precipitate
One of the easiest ways of separating casein from milk is to make the milk more acidic.
Ordinary white vinegar works very well because it contains dilute ethanoic acid, also known as acetic acid.
Milk normally has a pH somewhere around 6.5 to 6.8.
Casein proteins contain groups that can gain or lose H+ ions depending upon the pH.
As the pH falls towards approximately 4.6, casein reaches what chemists call its isoelectric point.
This is extremely important.
At the isoelectric point, the overall electrical charge on the protein is approximately zero.
The protein particles therefore repel each other much less strongly.
Instead of remaining distributed through the liquid, they begin sticking together.
The casein coagulates and precipitates.
Suddenly our smooth white milk begins separating into:
solid curds and liquid whey.
Anyone who has made cheese will recognise what is happening.
Precipitation, Coagulation and Cheese Chemistry
This gives us an opportunity to introduce several useful scientific words.
Precipitation
A substance that was previously dispersed or dissolved comes out of the liquid as a solid.
Coagulation
Small particles come together to form larger clumps or masses.
In this experiment the casein particles lose much of the electrostatic repulsion that normally keeps them apart.
They aggregate.
We see this as curds forming in the milk.
The remaining liquid is often called whey.
This is very similar to some of the chemistry used in food production.
But our objective is not cheese.
We are going to turn our casein into an adhesive.
What You Will Need
For a straightforward investigation you need:
about 100 mL of skimmed or semi-skimmed milk;
approximately 10-15 mL of white vinegar;
a small saucepan, beaker or heat-resistant container;
a thermometer if available;
a spoon or stirring rod;
filter paper, muslin, cheesecloth or a fine kitchen sieve;
paper towel;
sodium bicarbonate;
a small container for making the glue;
two wooden lolly sticks or pieces of wood for testing it.
Skimmed milk is particularly useful because the lower fat content generally gives a cleaner casein preparation.
Whole milk will still work, but the additional fat can make the separated material feel greasier.
Stage One — Warm the Milk
Measure approximately 100 mL of milk.
Warm it gently to around 40-50 degrees C.
It does not need to boil.
In fact, vigorous boiling is unnecessary and can complicate the experiment.
The warming simply helps the acid interact with the milk and allows the casein to coagulate relatively quickly.
Already there is a useful scientific point here.
Students often assume that whenever heat is used in an experiment, heat must be causing the chemical change.
Here it is mainly helping the process occur efficiently.
The acid is the crucial ingredient.
Stage Two — Add the Vinegar
Add approximately 10 mL of white vinegar while stirring gently.
The transformation can be remarkably rapid.
Within moments the previously smooth milk begins looking lumpy.
White solids appear.
The liquid surrounding them becomes more transparent and slightly yellowish.
Those solids contain the precipitated casein.
Add a little more vinegar if necessary until separation appears reasonably complete.
It is worth stopping at this stage and simply looking.
This is one of those practical experiments where the change is visually obvious enough that very little explanation is initially required.
Something fundamental has happened to the milk.
What Has the Acid Actually Done?
A very simplified explanation would be:
acid makes the casein precipitate.
But the underlying chemistry is more interesting.
Casein molecules contain acidic and basic groups.
The electrical charge carried by the proteins therefore depends upon the surrounding pH.
At normal milk pH, casein micelles carry sufficient charge to help keep them dispersed.
Adding ethanoic acid increases the concentration of H+ ions.
As the pH approaches the isoelectric point of casein, the overall charge decreases.
Repulsion between neighbouring protein particles becomes weaker.
They begin aggregating.
At the same time, acidification affects the calcium phosphate associated with the casein micelles, further destabilising their structure.
The result is the spectacular coagulation that we can actually see.
Is This Just Protein Denaturation?
Students may immediately think of cooking an egg.
Heating egg white causes proteins to unfold and form a solid network.
That is commonly described as protein denaturation.
Casein precipitation is slightly different.
Caseins do not have the same tightly folded structures as many other proteins.
Here, reducing the electrical charge and destabilising the casein micelles is especially important.
So although the words coagulation and denaturation are sometimes used rather loosely in everyday explanations, the chemistry deserves a little more care.
What we are primarily observing is acid-induced casein precipitation and aggregation.
Stage Three — Separate the Casein
Pour the mixture through filter paper, muslin or a fine sieve.
The liquid whey passes through.
The solid casein remains behind.
Press the casein gently with paper towel to remove as much liquid as possible.
You can rinse the curds with a little clean water and filter them again if you want to remove some of the remaining acid and soluble material.
Eventually you should have something resembling a soft white paste or crumbly putty.
At this stage it still does not look particularly promising as glue.
But we have now separated a natural polymer from milk.
Stage Four — Turn the Casein into Glue
Transfer the casein to a small container.
Add a small amount of sodium bicarbonate.
Start with perhaps a quarter of a teaspoon for casein obtained from around 100 mL of milk.
Mix thoroughly.
You may notice some gentle fizzing.
This occurs because sodium bicarbonate reacts with remaining acid.
A simplified ionic equation is:
H+ + HCO3- -> CO2 + H2O
The carbon dioxide produces the bubbles.
The bicarbonate also raises the pH.
This helps transform our acidic casein curds into a smoother and more usable adhesive paste.
Add a few drops of water if necessary.
The objective is not to produce a thin liquid.
You want something resembling a thick glue.
Why Should Protein Work as Glue?
This is perhaps the most interesting question in the entire experiment.
Why should something extracted from milk stick pieces of wood together?
Proteins are enormous molecules.
Casein molecules contain many different chemical groups capable of interacting with other substances.
When casein glue is spread across a surface, it can make close contact with microscopic irregularities in the material.
At the molecular level there can be interactions including:
hydrogen bonding;
electrostatic interactions;
attraction between polar groups;
and mechanical interlocking with pores and rough surfaces.
As water leaves the adhesive during drying, the casein molecules become increasingly concentrated.
Eventually they form a solid protein-rich layer between the two surfaces.
We have effectively created a natural polymer adhesive.
Test the Glue
A scientific investigation should not finish with:
"It looks like glue."
We should test whether it actually works.
Take two wooden lolly sticks.
Overlap them by perhaps 2 cm.
Spread approximately the same amount of casein glue over the overlapping region.
Clamp them together or place a small weight on top while the adhesive dries.
Leave them for several hours, preferably overnight.
Then try pulling them apart.
The result can be surprisingly convincing.
Turn It into a Proper Investigation
This experiment becomes much more interesting when students begin changing variables.
For example:
Does milk type matter?
Compare:
skimmed milk;
semi-skimmed milk;
whole milk.
Keep everything else constant.
Which produces the most casein?
Which produces the strongest glue?
How Much Casein Can You Obtain?
You can also turn the practical into a quantitative experiment.
Weigh a clean dry filter paper before filtration.
Collect the casein and allow it to dry thoroughly.
Weigh everything again.
Calculate:
Mass of casein = final mass - mass of filter paper
You could then calculate percentage yield relative to the mass of milk used:
Percentage yield = mass of dry casein / mass of milk x 100
Students should be careful with their interpretation.
Milk contains plenty of water, so we are not expecting an enormous percentage yield.
Does the Amount of Acid Matter?
Try adding different quantities of vinegar to identical volumes of milk.
For example:
2 mL;
5 mL;
10 mL;
15 mL;
20 mL.
Measure the mass of casein obtained.
At first, increasing the acid concentration should promote more complete precipitation.
Eventually, however, adding more acid should produce little additional benefit.
This is a useful demonstration of an important experimental principle:
more reagent does not necessarily mean more product indefinitely.
Measure the pH
If you have a pH meter or suitable pH probe, the experiment becomes considerably more informative.
Measure the starting pH of the milk.
Then add vinegar gradually while monitoring the pH.
Watch carefully as coagulation becomes extensive near the casein isoelectric region.
This converts what looks like an elementary kitchen experiment into a very good piece of analytical chemistry.
You can plot:
mass of casein precipitated against pH
or perhaps:
turbidity against pH
if suitable sensors are available.
Which Acid Works Best?
Another extension would be to compare different food-safe acids.
You might investigate:
white vinegar;
lemon juice;
citric acid solution.
Use solutions of comparable acidity where possible.
Students could investigate whether the type of acid matters or whether the principal factor is simply the pH achieved.
This is where experimental design becomes important.
If one sample receives much more acid than another, we cannot confidently say that differences arose because the acids themselves were different.
How Strong Is Our Milk Glue?
The most enjoyable extension may be engineering rather than chemistry.
Prepare several identical wooden joints.
Glue each pair using a different adhesive.
For example:
casein glue;
PVA glue;
flour paste;
starch adhesive.
Allow each sample to dry for the same length of time.
Then gradually add mass until the joint fails.
A simple results table might contain:
| Adhesive | Overlap area | Drying time | Maximum load before failure |
|---|---|---|---|
| Casein | 4 cm2 | 24 h | ... |
| PVA | 4 cm2 | 24 h | ... |
| Flour paste | 4 cm2 | 24 h | ... |
Now students are not simply making glue.
They are carrying out materials testing.
Be Careful About What You Call "Strong"
Suppose one glue supports a heavier mass than another.
Is that enough to declare it better?
Perhaps not.
We might also ask:
How long did it take to dry?
Was the joint waterproof?
Did the glue remain flexible?
Did it become brittle?
How easily could it be applied?
How long could it be stored?
Did it stick better to wood than plastic?
What happened in humid conditions?
Real engineering decisions rarely depend upon a single measurement.
The "best" material depends upon what we want the material to do.
A Glue with a Long History
Casein adhesives are not simply a classroom curiosity.
Before modern synthetic glues became widespread, casein-based adhesives were important materials for woodworking and plywood manufacture.
They offered a way of creating useful adhesives from naturally occurring proteins.
Modern synthetic adhesives such as PVA, epoxies and polyurethane products have largely replaced casein glue for many applications because they can offer better consistency, durability and water resistance.
Nevertheless, making casein glue gives students a glimpse of an earlier form of materials technology.
It also challenges an assumption we make increasingly often:
that useful manufactured materials must begin with petroleum or sophisticated industrial chemicals.
Nature already produces extraordinarily complicated polymers.
Sometimes chemistry is about learning how to separate and use them.
Milk, Cheese and Glue Are Connected by Chemistry
One of my favourite features of this experiment is the way it links apparently unrelated objects.
Milk.
Cheese.
Protein.
Glue.
They appear to belong to completely different worlds.
But at molecular level the connections become obvious.
Cheese making relies upon manipulating milk proteins.
Our glue-making experiment does something related, but instead of preparing food we deliberately recover the protein as a functional material.
This is one of the strengths of practical science.
The divisions between "food science", "chemistry" and "materials science" start disappearing.
There is simply matter — and what happens when we alter its conditions.
A Good Experiment for Discussing Colloids
Milk also gives us an opportunity to discuss something that receives surprisingly little attention in school science:
colloids.
Not every mixture is simply a solution or a suspension.
Milk contains extremely small particles dispersed through another substance.
Its proteins and fats interact with water in complicated ways.
Colloids are everywhere.
Examples include:
milk;
mayonnaise;
fog;
smoke;
paint;
shaving foam;
gelatin;
many cosmetics;
and numerous medicines.
Changing the conditions surrounding a colloid can cause it to become unstable.
Our precipitating casein is a beautiful example.
Common Problems
Nothing seems to happen
The milk may not be sufficiently warm, or you may not have added enough acid.
Add vinegar gradually while stirring.
The mixture is very greasy
You may be using milk with a high fat content.
Try skimmed milk.
The casein glue is too runny
Too much water has probably been added.
Add water only a few drops at a time.
The glue is too crumbly
Mix the casein more thoroughly with a small quantity of sodium bicarbonate and a few drops of water.
The joint seems weak
Allow considerably longer for drying.
Casein adhesive needs water to evaporate before maximum strength develops.
Also check that your surfaces are clean and make reasonably close contact.
Safety
Although this is a comparatively low-risk practical, normal laboratory precautions still apply.
Do not eat or drink laboratory materials.
Milk proteins can cause allergic reactions in people with milk allergies.
Take care when heating liquids.
Do not boil the milk unnecessarily.
Wash hands after the experiment.
Clean surfaces and equipment afterwards.
Once milk has been used experimentally, treat it as laboratory material rather than food.
Do not store homemade casein glue for long periods. Make a fresh batch when required.
Young students should carry out heating and chemical handling with appropriate adult supervision.
What Students Are Really Learning
At first sight, this might appear to be a novelty experiment.
"Make glue from milk."
But scientifically it contains far more depth than that description suggests.
Students encounter:
Biology:
Proteins and their properties.
Chemistry:
Acids, pH, neutralisation, precipitation and molecular charge.
Physics:
Intermolecular forces and material behaviour.
Materials science:
Polymers, adhesives and mechanical testing.
Experimental science:
Variables, controls, measurement, repeatability and data interpretation.
That combination makes it particularly valuable.
A good practical does not merely illustrate something students already know.
It gives them something new to think about.
From Breakfast to Materials Science
Perhaps the most memorable moment comes at the very beginning.
You start with an ordinary glass of milk.
Add a little acid.
Suddenly the liquid separates.
Filter it.
Treat the solid.
Spread it between two pieces of wood.
The following day those pieces may be firmly attached.
Nothing magical has occurred.
We have simply changed the conditions surrounding a naturally occurring polymer.
But that is precisely why the experiment is so satisfying.
Science allows us to look at an everyday substance and ask a completely different question.
Not:
"Can I drink this?"
but:
"What is actually inside it, how can I separate those substances, and what else could they do?"
That change of perspective is at the heart of good science.
Milk is not merely milk.
It is water, sugars, minerals, fats and proteins assembled into an extraordinarily complicated material.
And hidden among those proteins is casein — waiting for a little chemistry to turn breakfast into glue.


